Jonikas Lab



Jonikas Lab member names are bolded.


A Rubisco-binding protein is required for normal pyrenoid number and starch sheath morphology in Chlamydomonas reinhardtii

*Itakura AK, *Chan KX, Atkinson N, Pallesen L, Wang L, Reeves G, Patena W, Caspari O, Roth R, Goodenough U, McCormick AJ, Griffiths H#, & Jonikas MC#.

*Equal contribution #Corresponding authors

Proceedings of the National Academy of Sciences (2019) 116: 18445-18454.

PMID: 31455733


Phase separation in biology and disease-a symposium report

Cable J, Brangwynne C, Seydoux G, Cowburn D, Pappu RV, Castañeda CA, Berchowitz LE, Chen Z, Jonikas M, Dernburg A, Mittag T, Fawzi NL.

Annals of the New York Academy of Sciences (2019) 1452: 3-11.

PMID: 31199001


A genome-wide algal mutant library reveals a global view of genes required for eukaryotic photosynthesis

Li X, Patena W, Fauser F, Jinkerson RE, Saroussi S, Meyer MT, Ivanova N, Robertson JM, Yue R, Zhang R, Vilarrasa-Blasi J, Wittkopp TM, Ramundo S, Blum SR, Goh A, Laudon M, Srikumar T, Lefebvre PA, Grossman AR, & Jonikas MC.

Nature Genetics (2019) 51:627-635

PMID: 30886426


Effects of microcompartmentation on flux distribution and metabolic pools in Chlamydomonas reinhardtii chloroplasts

Küken A, Sommer F, Yaneva-Roder L, Mackinder LC, Höhne M, Geimer S, Jonikas MC, Schroda M, Stitt M, Nikoloski Z, & Mettler-Altmann T.

eLife (2018) doi: 10.7554/eLife.37960.

PMID: 30306890


A Spatial Interactome Reveals the Protein Organization of the Algal CO2-Concentrating Mechanism

Mackinder LCM, Chen C, Leib RD, Patena W, Blum SR, Rodman M, Ramundo S, Adams CM, & Jonikas MC.

Cell (2017) 171: 133-147.

PMID: 28938113

Highlighted on the cover of Cell and in a Cell Preview by Jean-David Rochaix.


The Eukaryotic CO2-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization

Freeman ES, Xu B†, Kuhn Cuellar L†, Martinez-Sanchez A, Schaffer M, Strauss M, Cartwright HN, Ronceray P, Plitzko JM, Förster F, Wingreen NS#, Engel BD#, Mackinder LCM‡, & Jonikas MC‡#.

†Equal contribution
‡Equal contribution

#Corresponding authors

Cell (2017) 171: 148-162.

PMID: 28938114

Highlighted in a Research Highlight in Nature Plants.


A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle

Mackinder LCM, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, Caspari O, Freeman Rosensweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Mühlhaus T, Schroda M, Goodenough U, Stitt M, Griffiths H, & Jonikas MC.

Proceedings of the National Academy of Sciences U S A (2016) 113: 5958-63.

PMID: 27166422


Regulation and levels of the thylakoid K+ antiporter KEA3 shape the dynamic response of photosynthesis in fluctuating light.

Armbruster U, Leonelli L, Correa Galvis V, Strand D, Quinn EH, Jonikas MC, & Niyogi KK.

Plant and Cell Physiology (2016) 57: 1557-1567

PMID: 27335350


High-throughput genetics strategies for identifying new components of lipid metabolism in the green alga Chlamydomonas reinhardtii

Li X & Jonikas MC.

Chapter 10 in Lipids in Plant and Algae Development, Y. Nakamura, Y. Li-Beisson (eds.), (2016) Springer.

PMID: 27023238


An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii.

*Li X, *Zhang R, *Patena W, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre P, Fitz-Gibbon ST, Grossman AR, & Jonikas MC.

*Equal contribution

The Plant Cell. (2016) 28: 367-87.

PMID: 26764374


Critical role of Chlamydomonas reinhardtii ferredoxin-5 in maintaining membrane structure and dark metabolism.

*Yang W, *Wittkopp TM, Li X, Warakanont J, Dubini A, Catalanotti C, Kim RG, Nowack EC, Mackinder LC, Aksoy M, Page MD, D'Adamo S, Saroussi S, Heinnickel M, Johnson X, Richaud P, Alric J, Boehm M, Jonikas MC, Benning C, Merchant SS, Posewitz MC, & Grossman AR.

*Equal contribution

Proceedings of the National Academy of Sciences U S A (2015) 112:14978-83.

PMID: 26627249

Introducing an algal carbon-concentrating mechanism into higher plants: location and incorporation of key components.

Atkinson N, Feike D, Mackinder LC, Meyer MT, Griffiths H, Jonikas MC, Smith AM, & McCormick AJ.

Plant Biotechnology Journal (2015) 14:1302-1315

PMID: 26538195

Molecular techniques to interrogate and edit the Chlamydomonas nuclear genome.

Jinkerson RE & Jonikas MC.
The Plant Journal (2015) 82: 393–412.

PMID: 25704665


A fluorescence-activated cell sorting-based strategy for rapid isolation of high-lipid Chlamydomonas mutants.

Terashima M, Freeman ES, Jinkerson RE, & Jonikas MC.
The Plant Journal (2015) 81: 147-59.

PMCID: PMC4280329

Ion antiport accelerates photosynthetic acclimation in fluctuating light environments.

Armbruster U, Carrillo LR, Venema K, Pavlovic L, Schmidtmann E, Kornfeld A, Jahns P, Berry JA, Kramer DM, & Jonikas MC.

Nature Communications (2014) 5: 5439.

PMCID: PMC4243252


Alternative Acetate Production Pathways in Chlamydomonas reinhardtii during Dark Anoxia and the Dominant Role of Chloroplasts in Fermentative Acetate Production.

Yang W, Catalanotti C, D'Adamo S, Wittkopp TM, Ingram-Smith CJ, Mackinder L, Miller TE, Heuberger AL, Peers G, Smith KS, Jonikas MC, Grossman AR, & Posewitz MC.

The Plant Cell (2014) 26: 4499-4518.

PMCID: PMC4277214


Waking sleeping algal cells.

Li X, Umen JG, & Jonikas MC.

Proceedings of the National Academy of Sciences U S A (2014) 111: 15610-15611.
PMCID: PMC4226088


Actin is required for IFT regulation in Chlamydomonas reinhardtii.

Avasthi P, Onishi M, Karpiak J, Yamamoto R, Mackinder L, Jonikas MC, Sale WS, Shoichet B, Pringle JR, & Marshall WF.

Current Biology. (2014) 24: 2025-2032.
PMCID: PMC4160380


High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA.

*Zhang R, *Patena W, Armbruster U, Gang SS, Blum SR, & Jonikas MC.

*Equal contribution

The Plant Cell (2014) 26: 1398-1409.

PMCID: PMC4036561


Martin's previous work:


The ER membrane protein complex interacts cotranslationally to enable biogenesis of multipass membrane proteins.

*Shurtleff MJ, *Itzhak DN, Hussmann JA, Schirle Oakdale NT, Costa EA, Jonikas M, Weibezahn J, Popova KD, Jan CH, Sinitcyn P, Vembar SS, Hernandez H, Cox J, Burlingame AL, Brodsky JL, Frost A, Borner GH, & Weissman JS.

*Equal contribution

eLife (2018) doi: 10.7554/eLife.37018.

PMID: 29809151


Automated identification of pathways from quantitative genetic interaction data.

Battle A, Jonikas MC, Walter P, Weissman JS, & Koller D.

Molecular Systems Biology (2010) 6: 379.

PMCID: PMC2913392


J domain co-chaperone specificity defines the role of BiP during protein translocation.

Vembar SS, Jonikas MC, Hendershot LM, Weissman JS, & Brodsky JL.

Journal of Biological Chemistry (2010) 285: 22484-22494.

PMCID: PMC2903355


Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum.

Jonikas MC, Collins SR, Denic V, Oh E, Quan EM, Schmid V, Weibezahn J, Schwappach B, Walter P, Weissman JS, & Schuldiner M.

Science (2009) 323: 1693-1697.

PMCID: PMC2877488


Identification of yeast proteins necessary for cell-surface function of a potassium channel.

Haass FA, Jonikas M, Walter P, Weissman JS, Jan YN, Jan LY, & Schuldiner M.

Proceedings of the National Academy of Sciences U S A (2007) 104: 18079-18084.

PMCID: PMC2084299