New tools provide new opportunities.
We have generated a genome-wide collection of 60,000 green algal insertion mutants as a powerful resource for the research community [1][2][3], and have started to use them to connect genotypes to phenotypes on a massive scale [4] (mutants can be searched and ordered here). We have developed pipelines for high-throughput characterization of protein localization and protein-protein interactions [5] that reveal key clues to the functions of uncharacterized proteins.
We now seek to enable the systematic placement of genes into pathways and the discovery of novel pathways by leveraging high-throughput genetics, protein localization, and protein-protein interactions.